ID   HOP_HUMAN               Reviewed;          73 AA.
AC   Q9BPY8; A8K0Z2; E9PB55; G3V294; Q8N0V6; Q96CI1;
DT   01-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   26-FEB-2020, entry version 146.
DE   RecName: Full=Homeodomain-only protein;
DE   AltName: Full=Lung cancer-associated Y protein;
DE   AltName: Full=Not expressed in choriocarcinoma protein 1;
DE   AltName: Full=Odd homeobox protein 1;
GN   Name=HOPX; Synonyms=HOD, HOP, LAGY, NECC1, OB1;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
RC   TISSUE=Heart;
RX   PubMed=14516659; DOI=10.1016/s0925-4773(03)00090-x;
RA   Adu J., Leong F.T., Smith N.R., Leek J.P., Markham A.F., Robinson P.A.,
RA   Mighell A.J.;
RT   "Expression of mOb1, a novel atypical 73 amino acid K50-homeodomain
RT   protein, during mouse development.";
RL   Mech. Dev. 119:S43-S47(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, AND POSSIBLE
RP   INVOLVEMENT IN CHORIOCARCINOMA.
RX   PubMed=12573257; DOI=10.1016/s0888-7543(02)00011-3;
RA   Asanoma K., Matsuda T., Kondo H., Kato K., Kishino T., Niikawa N., Wake N.,
RA   Kato H.;
RT   "NECC1, a candidate choriocarcinoma suppressor gene that encodes a
RT   homeodomain consensus motif.";
RL   Genomics 81:15-25(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, AND POSSIBLE
RP   INVOLVEMENT IN LUNG CANCER.
RX   PubMed=12759545; DOI=10.1159/000070306;
RA   Chen Y., Petersen S., Pacyna-Gengelbach M., Pietas A., Petersen I.;
RT   "Identification of a novel homeobox-containing gene, LAGY, which is
RT   downregulated in lung cancer.";
RL   Oncology 64:450-458(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Brain;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15815621; DOI=10.1038/nature03466;
RA   Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA   Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA   Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA   Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA   Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA   Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA   Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA   Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA   Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA   McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA   Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA   Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA   Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA   Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA   Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA   Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA   Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA   Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA   Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA   Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA   Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA   Wilson R.K.;
RT   "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT   4.";
RL   Nature 434:724-731(2005).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=B-cell;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [7]
RP   INVOLVEMENT IN HEAD AND NECK SQUAMOUS CELL CARCINOMA.
RX   PubMed=15213722; DOI=10.1038/sj.bjc.6601952;
RA   Lemaire F., Millon R., Muller D., Rabouel Y., Bracco L., Abecassis J.,
RA   Wasylyk B.;
RT   "Loss of HOP tumour suppressor expression in head and neck squamous cell
RT   carcinoma.";
RL   Br. J. Cancer 91:258-261(2004).
RN   [8]
RP   INVOLVEMENT IN ORAL SQUAMOUS CELL CARCINOMA.
RX   PubMed=15381369; DOI=10.1016/j.cancergencyto.2004.01.026;
RA   Toruner G.A., Ulger C., Alkan M., Galante A.T., Rinaggio J., Wilk R.,
RA   Tian B., Soteropoulos P., Hameed M.R., Schwalb M.N., Dermody J.J.;
RT   "Association between gene expression profile and tumor invasion in oral
RT   squamous cell carcinoma.";
RL   Cancer Genet. Cytogenet. 154:27-35(2004).
RN   [9]
RP   FUNCTION, AND INTERACTION WITH HSPA1A; HSPA1B AND HSPA8.
RX   PubMed=27708256; DOI=10.1038/ncomms12882;
RA   Seo J.H., Park J.H., Lee E.J., Vo T.T., Choi H., Kim J.Y., Jang J.K.,
RA   Wee H.J., Lee H.S., Jang S.H., Park Z.Y., Jeong J., Lee K.J., Seok S.H.,
RA   Park J.Y., Lee B.J., Lee M.N., Oh G.T., Kim K.W.;
RT   "ARD1-mediated Hsp70 acetylation balances stress-induced protein refolding
RT   and degradation.";
RL   Nat. Commun. 7:12882-12882(2016).
CC   -!- FUNCTION: Atypical homeodomain protein which does not bind DNA and is
CC       required to modulate cardiac growth and development. Acts via its
CC       interaction with SRF, thereby modulating the expression of SRF-
CC       dependent cardiac-specific genes and cardiac development. Prevents SRF-
CC       dependent transcription either by inhibiting SRF binding to DNA or by
CC       recruiting histone deacetylase (HDAC) proteins that prevent
CC       transcription by SRF. Overexpression causes cardiac hypertrophy (By
CC       similarity). May act as a tumor suppressor. Acts as a co-chaperone for
CC       HSPA1A and HSPA1B chaperone proteins and assists in chaperone-mediated
CC       protein refolding (PubMed:27708256). {ECO:0000250|UniProtKB:Q8R1H0,
CC       ECO:0000269|PubMed:27708256}.
CC   -!- SUBUNIT: Interacts with serum response factor (SRF). Component of a
CC       large complex containing histone deacetylases such as HDAC2 (By
CC       similarity). Interacts with the acetylated forms of HSPA1A and HSPA1B.
CC       Interacts with HSPA8 (PubMed:27708256). {ECO:0000250|UniProtKB:Q8R1H0,
CC       ECO:0000269|PubMed:27708256}.
CC   -!- INTERACTION:
CC       Q99081:TCF12; NbExp=3; IntAct=EBI-10295883, EBI-722877;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q8R1H0}. Cytoplasm
CC       {ECO:0000250|UniProtKB:Q8R1H0}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=4;
CC       Name=1; Synonyms=A;
CC         IsoId=Q9BPY8-1; Sequence=Displayed;
CC       Name=2; Synonyms=B;
CC         IsoId=Q9BPY8-2; Sequence=VSP_012659;
CC       Name=3;
CC         IsoId=Q9BPY8-3; Sequence=VSP_047290;
CC       Name=4;
CC         IsoId=Q9BPY8-4; Sequence=VSP_047290, VSP_012659;
CC   -!- TISSUE SPECIFICITY: Widely expressed. Expressed in the heart, brain,
CC       placenta, lung, skeletal and smooth muscles, uterus, urinary bladder,
CC       kidney and spleen. Down-regulated in some types of cancer such as lung
CC       cancer, choriocarcinoma, head and neck squamous cell carcinoma and oral
CC       squamous cell carcinoma. {ECO:0000269|PubMed:12573257,
CC       ECO:0000269|PubMed:12759545}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF492675; AAM46827.1; -; mRNA.
DR   EMBL; AF492676; AAM46828.1; -; mRNA.
DR   EMBL; AF492677; AAM46829.1; -; mRNA.
DR   EMBL; AF492678; AAM46830.1; -; mRNA.
DR   EMBL; AF492679; AAM46831.1; -; mRNA.
DR   EMBL; AF492680; AAM46832.1; -; mRNA.
DR   EMBL; AF492681; AAM46833.1; -; mRNA.
DR   EMBL; AB059408; BAB40926.1; -; mRNA.
DR   EMBL; AB059409; BAB40927.1; -; mRNA.
DR   EMBL; AB059410; BAB40928.1; -; mRNA.
DR   EMBL; AF454763; AAL56613.1; -; mRNA.
DR   EMBL; AK289707; BAF82396.1; -; mRNA.
DR   EMBL; AC108215; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC014225; AAH14225.1; -; mRNA.
DR   CCDS; CCDS3507.1; -. [Q9BPY8-1]
DR   CCDS; CCDS47062.1; -. [Q9BPY8-3]
DR   CCDS; CCDS54767.1; -. [Q9BPY8-4]
DR   RefSeq; NP_001138931.1; NM_001145459.1. [Q9BPY8-1]
DR   RefSeq; NP_001138932.1; NM_001145460.1. [Q9BPY8-4]
DR   RefSeq; NP_115884.4; NM_032495.5. [Q9BPY8-3]
DR   RefSeq; NP_631957.1; NM_139211.4. [Q9BPY8-1]
DR   RefSeq; NP_631958.1; NM_139212.3. [Q9BPY8-1]
DR   RefSeq; XP_016864218.1; XM_017008729.1.
DR   RefSeq; XP_016864219.1; XM_017008730.1. [Q9BPY8-1]
DR   RefSeq; XP_016864220.1; XM_017008731.1. [Q9BPY8-1]
DR   RefSeq; XP_016864221.1; XM_017008732.1. [Q9BPY8-1]
DR   RefSeq; XP_016864222.1; XM_017008733.1. [Q9BPY8-1]
DR   RefSeq; XP_016864223.1; XM_017008734.1. [Q9BPY8-1]
DR   SMR; Q9BPY8; -.
DR   BioGrid; 124117; 12.
DR   IntAct; Q9BPY8; 4.
DR   MINT; Q9BPY8; -.
DR   STRING; 9606.ENSP00000450527; -.
DR   iPTMnet; Q9BPY8; -.
DR   PhosphoSitePlus; Q9BPY8; -.
DR   BioMuta; HOPX; -.
DR   DMDM; 60392394; -.
DR   jPOST; Q9BPY8; -.
DR   MassIVE; Q9BPY8; -.
DR   PaxDb; Q9BPY8; -.
DR   PeptideAtlas; Q9BPY8; -.
DR   PRIDE; Q9BPY8; -.
DR   ProteomicsDB; 19147; -.
DR   ProteomicsDB; 32566; -.
DR   ProteomicsDB; 78595; -. [Q9BPY8-1]
DR   ProteomicsDB; 78596; -. [Q9BPY8-2]
DR   DNASU; 84525; -.
DR   Ensembl; ENST00000317745; ENSP00000315198; ENSG00000171476. [Q9BPY8-1]
DR   Ensembl; ENST00000337881; ENSP00000337330; ENSG00000171476. [Q9BPY8-1]
DR   Ensembl; ENST00000381255; ENSP00000370654; ENSG00000171476. [Q9BPY8-1]
DR   Ensembl; ENST00000381260; ENSP00000370659; ENSG00000171476. [Q9BPY8-2]
DR   Ensembl; ENST00000420433; ENSP00000396275; ENSG00000171476. [Q9BPY8-3]
DR   Ensembl; ENST00000503639; ENSP00000424101; ENSG00000171476. [Q9BPY8-1]
DR   Ensembl; ENST00000508121; ENSP00000422175; ENSG00000171476. [Q9BPY8-1]
DR   Ensembl; ENST00000553379; ENSP00000452340; ENSG00000171476. [Q9BPY8-1]
DR   Ensembl; ENST00000554144; ENSP00000450527; ENSG00000171476. [Q9BPY8-4]
DR   Ensembl; ENST00000555760; ENSP00000452098; ENSG00000171476. [Q9BPY8-1]
DR   Ensembl; ENST00000556376; ENSP00000451794; ENSG00000171476. [Q9BPY8-1]
DR   Ensembl; ENST00000556614; ENSP00000452003; ENSG00000171476. [Q9BPY8-1]
DR   GeneID; 84525; -.
DR   KEGG; hsa:84525; -.
DR   UCSC; uc003hbz.3; human. [Q9BPY8-1]
DR   CTD; 84525; -.
DR   DisGeNET; 84525; -.
DR   GeneCards; HOPX; -.
DR   HGNC; HGNC:24961; HOPX.
DR   HPA; CAB018632; -.
DR   HPA; HPA030180; -.
DR   MIM; 607275; gene.
DR   neXtProt; NX_Q9BPY8; -.
DR   OpenTargets; ENSG00000171476; -.
DR   PharmGKB; PA162391564; -.
DR   eggNOG; KOG0490; Eukaryota.
DR   eggNOG; ENOG410YIJ3; LUCA.
DR   GeneTree; ENSGT00390000017143; -.
DR   HOGENOM; CLU_193231_0_0_1; -.
DR   InParanoid; Q9BPY8; -.
DR   OMA; KVSKHPD; -.
DR   OrthoDB; 1567787at2759; -.
DR   PhylomeDB; Q9BPY8; -.
DR   SIGNOR; Q9BPY8; -.
DR   ChiTaRS; HOPX; human.
DR   GeneWiki; HOPX; -.
DR   GenomeRNAi; 84525; -.
DR   Pharos; Q9BPY8; Tbio.
DR   PRO; PR:Q9BPY8; -.
DR   Proteomes; UP000005640; Chromosome 4.
DR   RNAct; Q9BPY8; protein.
DR   Bgee; ENSG00000171476; Expressed in caput epididymis and 224 other tissues.
DR   Genevisible; Q9BPY8; HS.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0000790; C:nuclear chromatin; ISA:NTNU_SB.
DR   GO; GO:0005634; C:nucleus; IDA:MGI.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISA:NTNU_SB.
DR   GO; GO:0051131; P:chaperone-mediated protein complex assembly; IDA:CAFA.
DR   GO; GO:0045596; P:negative regulation of cell differentiation; IDA:MGI.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0001829; P:trophectodermal cell differentiation; IDA:MGI.
DR   CDD; cd00086; homeodomain; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR039162; HOPX.
DR   PANTHER; PTHR21408; PTHR21408; 1.
DR   Pfam; PF00046; Homeodomain; 1.
DR   SMART; SM00389; HOX; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Cytoplasm; Developmental protein; Homeobox; Nucleus;
KW   Proto-oncogene; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..73
FT                   /note="Homeodomain-only protein"
FT                   /id="PRO_0000049129"
FT   DNA_BIND        3..62
FT                   /note="Homeobox; atypical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   VAR_SEQ         1
FT                   /note="M -> MLIFLGCYRRRLEERAGTM (in isoform 3 and isoform
FT                   4)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_047290"
FT   VAR_SEQ         49..73
FT                   /note="KWFKQRLAKWRRSEGLPSECRSVTD -> GSDLISRSKIWHPESSPQREGYP
FT                   HDSLPCLAFDYFSLLPPQCKEMV (in isoform 2 and isoform 4)"
FT                   /evidence="ECO:0000303|PubMed:14516659"
FT                   /id="VSP_012659"
FT   CONFLICT        72
FT                   /note="T -> I (in Ref. 6; AAH14225)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        Q9BPY8-2:76
FT                   /note="P -> L (in Ref. 1; AAM46830/AAM46831)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   73 AA;  8260 MW;  CE65E4D2A8972022 CRC64;
     MSAETASGPT EDQVEILEYN FNKVDKHPDS TTLCLIAAEA GLSEEETQKW FKQRLAKWRR
     SEGLPSECRS VTD
//